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Cryo-ET Webinar Series

The National Network for Cryo-Electron Tomography Webinars are given weekly over Zoom every Thursday at 12:00pm CST from July 8, 2021 until August 12th, 2021.

Register to attend the talks live via Zoom at National Network for Cryo-Electron Tomography Webinar Series.

Webinar Archive

An introduction to cryo-ET and overview of the National Network for Cryo-ET

Date: 2021-07-08


Cryo-electron tomography (cryo-ET) has revolutionized biological research by providing nanometer-scale resolution 3D reconstructions of biological structures, such as purified complexes, viruses, bacteria and archaea, and eukaryotic cells. The National Network for Cryo-Electron Tomography is an NIH-funded consortium whose mission is to provide access to both the instrumentation and expert staff for advancing the original research of scientific investigators and for training those new to cryo-electron tomography. Join us in this webinar series to learn more about this network and about how you can access these world-class cryo-electron tomography resources in your research program. This webinar introduces the National Network for Cryo-ET Centers. Short introductions are given by each of the facility starting with NCITU, then CCET, SCSC, and MCCET.

High pressure freezing, the waffle method and cryo-FIB, Mykhailo Kopylov, NCITU

Date: 2021-07-15


Mykhailo Kopylov from the National Center for In-situ Tomographic Ultramicroscopy (NCITU) presents on the High-Pressure Freezing "waffle" method for vitrification of samples including mammalian cells, cell clumps like bacteria or yeasts, purified proteins, large cells and cell aggregates, and tissues with 10 um limit. He provides an overview of the problems for vitrification of samples and how the HPF waffle technique provides new sample workflows.

Correlative cryogenic light and electron microscopy and tomography, Gong-Her Wu, SCSC

Date: 2021-07-22


Cryo-electron tomography (cryo-ET) has revolutionized biological research by providing nanometer-scale resolution 3D reconstructions of biological structures, such as purified complexes, viruses, bacteria and archaea, and eukaryotic cells. Gong-Her Wu presents an overview of the imaging technologies and resolution ranges. This talk covers workflows for multi-scale cryo-correlative imaging of cells including confocal microscopy, cryo-EM, and cryo-FIB-SEM.

Preparation of select murine brain sections for HPF, cryo-CLEM, and cryo-ET, Michael H.B. Stowell, CCET

Date: 2021-07-29


Cryo-electron tomography (cryo-ET) has revolutionized biological research by providing nanometer-scale resolution 3D reconstructions of biological structures, such as purified complexes, viruses, bacteria and archaea, and eukaryotic cells. Michael H.B. Stowell of the CU Boulder Center for Cryo Electron Tomography will present on preparing murine brain sections with HPF, cryo-CLEM, and cryo-FIB techniques. The webinar provides a background on the isolation of tissues, embedding in agar, vibratome sectioning, and use of high-pressure freezing techniques.

Structural studies of virus infection using CLEM, FIB and cryo-ET by Jie E. Yang, MCCET

Date: 2021-08-12


Cryo-electron tomography (cryo-ET) has revolutionized biological research by providing nanometer-scale resolution 3D reconstructions of biological structures, such as purified complexes, viruses, bacteria and archaea, and eukaryotic cells. In this webinar, Jie provides an introduction to the techniques of CLEM, cryo-FIB, and cryo-ET with a focus on the cell-virus interactions. Jie presents on the structural studies of viral infections and the development of high-precision cryo-CLEM flexible workflows.

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